Finally, 0. In addition, the dissected tissues (heart, liver, spleen, lung and kidneys) were fixed in 4% paraformaldehyde, embedded in paraffin, and sliced into 4-μm thick sections. Unit price / per . ChE activity was normalized by protein concentration as determined by a Coomassie. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. Molecular Devices SpectraMax Plus 384 Microplate Reader *Parts Only* Tecan Sunrise Microplate Reader. The reaction was started by adding 10 µL of the enzyme solution (properly diluted) to 190 µL of 15 mM ONPG in 0. Click SoftMax Pro Software User Guide. After that, absornace were measured at 520 nm using using a microplate reader spectrophotometer VERSAmax (Molecular Devices). from Molecular Devices Corporation. VersaMax™ ELISA microplate reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 to 850 nm). VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 4 0112-0126B SafetyInformation InformationaboutthesafeuseoftheinstrumentfromMolecularDevices®includesan Get the most out of SoftMax Pro. Molecular Devices Corporation. Molecular Devices Corporation SpectraTest ABS1 Validation Plate Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1. Biosystems CytoFluor 4000 Multi Well Plate Reader $ 1,995. Also see: SoftMax® Pro Microplate Software User Manual Download Page. Then the absorbance at 570 nm was measured using a microplate reader (VersaMax, Molecular Devices, San Jose, CA, USA). The emodin thermoreversible PG was. Cell-free AB broth (22) of an overnight culture of the acylhomoserine lactone-defective V. Ensure data accuracy of your reader. After freeze-drying, the. 9000-0756. In addition, the dissected tissues (heart, liver, spleen, lung and kidneys) were fixed in 4% paraformaldehyde, embedded in paraffin, and sliced into 4-μm thick sections. 2. ®SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. +49-89/96-05-88-0 fax +49-89/9-62-02-34-5 Japan Nihon Molecular Devices Osaka Tel. 3 cells (6x105) were ocExtracts were placed in a 96-well plate in triplets and spectrophotometric readings were taken at 555 nm on a microplate reader (Versamax, Molecular Devices LLC, USA). Related products. Battery Backup Uninterruptible Power Supply (UPS) And Power Conditioner For MDS Sciex Molecular Devices VERSAmax Microplate Reader Quote Request. 68 $. Absorbance was determined at 490 nm on a microplate reader (VersaMax; Molecular Devices, Sunnyvale, CA, USA). Regular price $ 0. Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. VERSA MAX-LUNABLE, MOLECULAR DEVICES, MICROPLATE R. Manufacturer: Molecular Devices; Includes PC Workstation w/ Softmax Pro Software Wavelength Range (nm): 340–850 Wavelength Accuracy: < ±0. Sing a Microplate Reader (VersaMAx, Molecular Devices). The collaborative space brings customers and researchers into the lab to test automated workflows for organoid culturing and screening, with guidance from in-house. Absor-bance of the solution was measured using a micro plate Reader (VersaMax, Molecular Devices) at 570 nm. 17. SoftMax Pro. s. 5×10 3 cells were seeded in 96-well plates, treated with indicated inhibitors for 48 or 72 hours, analyzed 16 hours after BrdU addition, and absorbance measured at 450nm using a spectrophotometer plate reader (VersaMax, Molecular. Readings were taken every 15 s at 490 nm and the results were analysed using SOFT-MaxPro 4. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. 0 software (GraphPad). It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 0. Narrow bandwidths deliver increased accuracy and enhanced peak. It provides both rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. It provides both rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. measured over 60min using a microplate reader (VersaMax, Molecular Devices, Sunnyvale, CA) at a wavelength of 405nm. TECHNICAL NOTE. Manual for Molecular Devices versamax Laboratory Equipment (47 pages) Specifications: 1379/1379020-versamax. Tris base (100 μL of a 10 mM solution) was added to the wells to solubilize the bound SRB, and absorbance was then read at 515 nm on an automated microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA, USA). 0A-Sunnyvale, CA-Made in USASpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Reverse transcription-quantitative polymerase chain reaction To assess phospholipase C gamma 1 (PLCG1) and phospholi-pase C gamma 2 (PLCG2) mRNA levels in each. Battery Backup Uninterruptible Power Supply (UPS) And Power Conditioner For MDS Sciex Molecular Devices VERSAmax Microplate Reader Quote Request. This. The catalytic activity was calculated from the production rate of the hydrolyzed product, p-nitrophenol, by monitoring the absorption (405 nm) at room temperature. The plate was read at 570 nm after 5 min using Versamax (molecular devices, United States). +49-89-9620-2340 Fax +49-89-9620-2345 Japan Nihon Molecular Devices Tel. VersaMax ELISA Microplate Reader from Molecular Devices, LLC Description Enables precise and accurate detection at wavelengths in the visible spectrum without the use of. All cell lines were cultivated for 24 h in 96-well cell culture plates with an initial concentration of 1×10 5 cells/mL. 3, Molecular Devices). used. An aliquot of the extracts dissolved in DMSO with 80 μL of phosphate buffer (pH 6. NORTH AMERICA. After three times washing, the reaction was colored by substrate 3,3’,5,5’-Tetramethylbenzidine (TMB) (T0440, Sigma), and stopped by 2 N HCl. 0%, 0-2. ¨ Molecular Devices is a life-science technology innovator Our integrated hardware and software solutions enable automated workflows—from start to finish—for any research application. 00 Add to cart Molecular Devices Microplate Reader Spectramax 384 340PC $ 6,295. It was verified that absorbance values were proportional to the amount of a bound peroxidase-labeled antibody. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 2 0112-0126B ThisdocumentisprovidedtocustomerswhohavepurchasedMolecularDevicesequipment,software,SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Substrates and coenzymes provided and specified in the kit were added at 37°C after 1 min plate was read in a spectrophotometer (Versamax, Molecular Devices, San Jose, CA, USA) at 37°C every 1 min for 4 min. With optimized reagents, option to operate the. Taking into account the price differences between each seller and each country. +86-21-6887-8820 fax +86-21-6887-8890 Germany Molecular Devices GmbH Tel. 1 mL. MDC instruments including: SpectraMax®, VersaMax. 0A 2 Lines Fused, unplug before ser. ®ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. The catalytic activity was calculated from the production rate of the hydrolyzed product, p-nitrophenol, by monitoring the absorption (405 nm) at room temperature. P. SpectraMax® Microplate Reader User Manual Download Page. Spectrofluorometer (e. 5 million deaths worldwide have been caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (), along with high levels of unemployment and far-reaching supply chain issues in the world’s economy. 0A-Sunnyvale, CA-Made in USA SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. Used. The Molecular Devices SpectraMax XPS has an optical design that is the only multi-channel system that truly mimics a dual-beam spectrophotometer. The sections were stained with hematoxylin and eosin (H&E) and. Offer Ends In: 5 Days 05: 02: 03. SpectraMax Plus384, 190, 340PC384, and VersaMax Installation Guide Author: Molecular Devices Subject: Installation Guide Keywords: SpectraMax Versa and others Detection Platform Created Date: 5/17/2023 2:14:27 PM At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the VERSAmax Tunable Microplate Reader are warranted for a period of one (1) year from the original date of delivery. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. A series of 44 excipients were evaluated at six different concentrations in triplicate. The Molecular Devices VersaMax Microplate Reader is a high-performance laboratory instrument designed for fast and accurate measurements allowing for selection of up to two wavelengths at a time. Table of Contents. 5°C @ 37°C Well-to. Molecular Devices versamax Operator's Manual (47 pages) Tunable Microplate Reader. 00 Read more Molecular Devices Tunable Microplate Reader VersaMaxMicroplate reader with 450 nm filter, such as VersaMax (Molecular Devices, Sunnyvale, CA). Cell viability was represented by percentage values compared to a control and then used to calculate the number of viable cells. Molecular Devices Versamax; Molecular Devices Vmax; Molecular Devices Fmax Fluorescence; Packard Fluorocount Fluorescence; Packard Spectracount; Tecan Spectrafluor Plus Multilabel; Perkin Elmer HTS-7000 Plus Multilabel; Skatron Skanstacker 300 Washer; Berthold LB96P Luminescence;Finally, cells were incubated with 1% SDS for 2 h at RT to release the dye from the cells, and the cell proliferation rate was assessed by quantification of the amount of dye released from the cells using a spectrophotometer (VersaMax, Molecular Devices) at the wavelength of 570 nm. The signal was read on an ELISA plate reader (SpectraMax VersaMax, Molecular Devices, Sunnyvale, CA, USA). , St. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Florescence intensity was monitored on a Gemini XPS fluorescence microplate reader (Molecular Devices, Sunnyvale, CA, USA) set for 488 nm excitation and 520 nm emission. The activity was measured colorimetrically at 450 nm using a microplate reader (Versamax, Molecular Devices, Sunnyvale, CA, USA). BMG Labtech - THERMOstar. New and used Molecular Devices VersaMax's for sale on LabX. 0 OD Kinetic Read Time: 9 Second Minimum Interval Temperature Uniformity ±0. SoftMax Pro 7. Add to Cart. HepG2 cells were seeded into 6-well plates. A. In this study, we have prepared thermoreversible PG containing emodin with high emodin solubility and the gelation temperature suited to physiological use. ® SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. After 30-min incubation, the absorbance was measured at 405 nm wavelength using a spectrophotometer device (VersaMax Molecular Devices, Sunnyvale, CA, USA). Cells were treated with 150 μl of dimethylsulfoxide (DMSO) and absorbance was quantified at 540 nm using the microplate spectrophotometer system (VersaMax, Molecular Devices, Sunnyvale, CA, USA). The IC 50 value was generated from the log dose-response curves for cells using the Graphpad Prism version 9 for Windows (Graphpad Software, La Jolla, CA, USA). With its advanced features and user-friendly interface, the VersaMax is the perfect choice for a wide range of research. StakMax. The Molecular Devices VersaMax Absorbance Microplate Reader enables both precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility. reader (VersaMax, Molecular devices, San Jose, CA, USA). VERSA MAX-LUNABLE, MOLECULAR DEVICES, MICROPLATE R. Molecular Devices Corporation SpectraTest ABS1 Validation Plate Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1. Glossary of Terms. All animal experiments were performed in compliance with the relevant laws and. All samples were measured in triplicate. The optical density (OD) of the mixture was measured at 490 nm with a microplate absorbance reader (VersaMax (Molecular Devices), Shanghai, China). Monday to Friday 7am - 5pm Pacific Time. SpectraMax and VersaMax Plate Readers Operator s Manual -- 0112-0126 Rev A Molecular De. 5) containing 0. This version is compatible with the following instruments: SpectraMax® i3x Multi-Mode Detection Platform. Acetylcholinesterase activity was calculated as the rate of change of absorbance per minute during the linear portion of the reaction and normalized by protein concentration as determined by a Coomassie. For pinocytosis, cells in wells were washed in PBS and 5 mg/ml of HPTS dye (in PBS) added and incubated with gentle rocking at 30 °C for 120 min. 00 USD. Coat plate with 100 μL each of appropriately diluted standards (Table 1),. The system provides automation for up to 50 For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. +1 800-635-5577. VersaMax™ Tunable Microplate Reader, Molecular Devices. 0. In brief, mature 3T3-L1 cells were washed with DPBS, fixed with 10% formalin for 1 h at room temperature, washed once with 60%. 0 nm full width half maximum. Cet appareil ISM est confomre à la norme NMB-001 du Canada. A. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. SpectraTest ABS1 Absorbance Validation Plate. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0140 Rev. 00 Sale price $ 0. S. We would like to show you a description here but the site won’t allow us. 2 and 648. Molecular Devices Beijing Tel. EUROPE. The plate was read at 570 nm using an ELISA reader (VERSAMax, Molecular Devices, VWR, Philadelphia, PA, USA). Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. # SMP54-GXP- 10UL) Pyrochrome Assay. The absorbance was then determined by an enzyme-linked immunosorbent assay (ELISA) reader (VERSAmax; Molecular Devices, Sunnyvale, CA) at a wavelength of 405 nm. The absorbance at 570 nm was determined by VersaMax (Molecular Devices, CA) to estimate MTT-formazan production after 24, 48, and 72 hours of incubation to evaluate the proliferation rate. The product formed was then transferred to 96 well plate and the absorbance was measured at 540 nm using microplate reader (VersaMax™, Molecular Devices, CA, USA). The detection limit for rivaroxaban in this assay was 10 ng/mL. We use. 5 mM EDTA, 0. The cells were treated with 15, 25, 30, and 45 nm-particles in order to compare the cytotoxicities of different sized nanoparticles on the treated cells. Ann Arbor, MI, USA). The Molecular Devices VersaMax Tunable Microplate Reader for 96-well format uses a grating monochromator to select the wavelength, so the exact wavelength needed can. Spectrophotometers. While the cell covers about. The quantity of nitrite in the samples was calculated using the concentration of sodium nitrite as a standard. Molecular Devices Versamax Tunable Microplate Reader is available from The Lab World Group. The Molecular Devices VersaMax Absorbance Microplate Reader enables both precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility. (VersaMax, Molecular Devices, USA). Coat plate with 100 μL each of appropriately diluted standards (Table 1),. We would like to show you a description here but the site won’t allow us. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 11 0112-0126B Theguidelinesforcuvetteuseintheinstrumentarethesameasthosethatapplytoany high. For the MTS assay, the CellTiter 96® AQ ueous One Solution Cell Proliferation Assay kit was used following the manufacturer's instruction. Whether bacteria were partially or completely inactivated by bone cement. Molecular Devices versamax Operator's Manual (47 pages) Tunable Microplate Reader. Finally, a 5% glucose solution was added to the suspension of CPN and FCPN as a freeze-dried protective agent. View the supported Window operating systems for the different versions of the SoftMax Pro software. The robot-compatible VersaMax Reader is cost-effective—users never have to buy filters. Molecular Devices VersaMax Tunable Temperature range between 4°C above ambient to 45°C Digital controller and display Sold as-is The Molecular Devices VersaMax microplate spectrophotometer provides rapid and sensitive measurements for a variety of analytes across a wide range of concentrations. Turn the reader on and secure the USB cable. Read-out was performed within 30 minutes with a microtiter plate reader at 450 nm (Versamax, Molecular Devices). 003 OD ±1. Never order or replace filters to optimize an assay again! Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. $199. 8), 40 μL of tyrosinase, and 40 μL of L-DOPA were put in each well. Supplier: Molecular Devices. (Molecular Research Center, USA) according to the manufacturer’s. Softmax Pro 소프트웨어가 포함된 PC 워크스테이션 포함. S. SoftMax® Pro Software is the leading solution for microplate reader control and data analysis, where our exclusive INSIDER members help to ensure it remains the most innovative research tool available. the plate reader (model VERSAmax, Molecular Devices, USA) at 515 nm (A515). BMG Labtech - THERMOstar. Ann Arbor, MI, USA). E. ~ Bio-Tek ELx808 Microplate Reader $2,795. All cell lines were cultivated for 24 h in 96-well cell culture plates with an initial concentration of 1×10 5 cells/mL. 专为科研检测平台量身打造的灵活且强大的小型微孔板读板机（酶标仪），具备终点法和动力学检测模式，标准配置6块滤光片，覆盖蛋白定量、细胞活力、农药残留、ELISA实验等. One microgram of RNA was. Briefly, at 3 h before each of the desired time points, 10 µl of the MTS reagent. Plates were read using a microplate reader (VersaMax, Molecular Devices) at 595 nm. Optical Densitiy (OD) values were processed with a data reduction software (SoftMax Pro, Molecular Devices) using a 4-parameter logistic fitting algorithm to the standard curve, allowing the determination of antibody. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. used. Furthermore, VersaMax is reliable, which means more uptime and lower long-term cost of. The reaction in the wells was stopped by the addition of 50 μl of 1 M sulfuric acid, which turned the wells yellow. Regular price $ 0. ) for the next 36 h and provided a time-proliferation curve as a test result for each well of the microplate. Cell viability was analyzed using a cell counting kit assay (Dojindo). SpectraMax Plus384 SpectraMax 190 SpectraMax 340PC384 VersaMax Microplate Spectrophotometer Operator s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. To update the driver. 1700 +1 800. Finally, a 5% glucose solution was added to the suspension of CPN and FCPN as a freeze-dried protective agent. Product Overview VersaMax™ ELISA Microplate Reader Manufacturer Molecular Devices® | Available Worldwide Be the first to review this product Request Pricing Molecular Devices® Manufacturer Website. SpectraMax® i3 Multi-Mode Detection Platform. The primers for the human genes GAPDH, SOD1, SOD2, CAT, GPX1, CCS, PRDX6, FOXO3, CDKN2A,. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. The plates were immediately measured at 405 nm for 6 min using a microtiter plate reader (VersaMax; Molecular Devices, Sunnyvale). Microplate Reader Capability > UV - Vis Absorbance. +06-6399-8211 Fax +06-6399-8212 ©2002 Molecular Devices Corporation. The absorbance was measured at 450 nm using a microplate reader (VersaMax, Molecular Devices). (VersaMax, Molecular Devices, Sunnyvale, CA, US). For more sample throughput, the system can be easily integrated into a full robotic The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. We would like to show you a description here but the site won’t allow us. Molecular Devices VersaMax Tunable Microplate Reader. HT22 cells are derived from hippocampal neuronal HT4 cells. Then, the cultured cells were treated with different. High-Throughput Screening. The calibration curve was fitted to a sigmoid by the four-parameter method in the SoftMax Pro software (Molecular Devices), and allowed the calculation of the concentration of native Antigen 18A. Absorbance was read on a spectrophotometer (VersaMax, Molecular Devices) with SoftMax Pro GxP v5 software. Molecular Devices, LLC. treatments were then examined atλ=570 nm using VersaMax eliSa reader (Molecular devices, llc. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. The wells were visualized using o-phenylenediamine (OPD), and the absorbance was read with a multiwall spectrophotometer (VERSAmax, Molecular Devices, Sunnyvale, CA, USA) at 490 nm. Cell. Western Blot. At 48 h after transfection the cells were exposed to CoCl 2 (200 µM) or vehicle. 1 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 compliance to ensure data integrity. 온도 균일성 ±0. GABA concentrations in samples were calculated from calibration curves using the standard solutions. Model: VERSAmax Tunable Microplate Reader. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. If the computer is connected to the Internet: Select Search Automatically for updated driver software. The absorbance was assumed to be 100% for the control group. 4 (Molecular Devices cat. (Seongnam, Korea). EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Filterless Absorbance Microplate Readers provides fast & consistent results with steady temperature enabling a wide range of assays - ELISA reader, microbial. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The mouse hippocampal neuronal cell line HT22 is frequently used as an in vitro model to investigate the role of hippocampal cholinergic neurons in cognitive functions. Unit price / per . We would like to show you a description here but the site won’t allow us. Microplate Reader Shelf. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. OptiMax (Versamax non EXT) B0: Screenstation: all: Skanstacker: all: Skanwasher 300: all: Skanwasher 400: all: SpectraMax 190: N0:. Afterward, HUVECs were incubated with EPC-EVs, TFEB-EVs, or PBS for 24 h or 48 h. They were then pre-treated with indicated concentration of drugs for 1 h prior to being treated or not with 5-HT or serum. The mixture was incubate at 37 °C for 30 min, and then the reaction was stopped by the addition of 0. It features a dual-monochromator system, a large dynamic range, and a high-throughput capability. The SpectraTest® ABS1 Absorbance Validation. S. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein. Find regional support. Designed to provide the simplicity, flexibility and power required for advanced data analysis. 5 mg/mL) in the range of 5–100 μg per 0. Molecular devices The VersaMax Absorbance Microplate Reader costs between $5,500 and $6,600. Enzyme activity was. Wavelength Range. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. Our products enable scientists to improve productivity and efficiency in scientific discovery and research. Cell viabilities were presented as the ratio of the absorbance of treated cells to the absorbance of control cells cultured with an extract of HDPE. All experiments were repeated three times. No accessories are needed and SoftMax® Pro Software. Microplate assays involved a 96-microplate reader (VERSAmax; Molecular devices, Sunnydale, California, USA) used with flat-bottomed 96-well microplates (NUNC, Sigma Aldrich, UK). , Sunnyvale, CA). Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The narrowly focused light beam and optical design give you the same high performance with round bottom, flat bottom, or half area well plates. absorbance readers. Microwell plates, black (96-well) Rocking table with adjustable lifting frequency. Get pricing and purchase lab equipment and instrumentation today. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers begining with. MiR-21. 0200-6117. Taking into account the price differences between each seller and each country. Although this manual has been prepared with every precaution to ensure accuracy, Molecular DevicesThe amount of extracted crystal violet was measured at 595 nm using a microplate reader (VersaMax, Molecular Devices Co. Molecular Devices VersaMax Microplate Reader Molecular Devices VersaMax Microplate Reader Specifications. The SpectraTest® ABS1 Absorbance. Molecular Devices VersaMax Absorbance (UV/Vis) Elisa Microplate Reader For Sale for Plate Reader is complete with computer, SoftMa. The objectives of this study were to determine phytochemical compositions, chemiluminescence antioxidant activities, and neuroprotective effects on PC12 cells for water, methanol, and 95% ethanol extracts of the air-dried fruit of <i>Terminalia chebula</i> Retzius. absorbance readers. Software (optional), such as Softmax Pro (Molecular Devices). This reliable model allows for accurate wavelength detection in visible spectrum without the need for filters. JEG-3 cells were transfected with myc-tagged NDRG1 wild type or deletion mutants at the N- or C- terminal as depicted in Figure 5A. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. Used. Total. 1 M sodium phosphate buffer (pH 6. ¨ We would like to show you a description here but the site won’t allow us. The color was allowed to develop for 20 min in dark and the reaction was stopped with 2. ® US HEADQUARTERS. harveyi BB152 was used as the AI-2-producing positive control (prepared as reported (11)). The conversion of L-DOPA to dopachrome was allowed to proceed for 40 min at 30 °C in a microplate reader (Versamax; Molecular Devices). The absorbance was measured at 450 nm with 650 nm as a reference on a VersaMax (Molecular Devices, Sunnyvale, CA) and a standard curve was plotted using a 4-parametric mathematical fit model in. for cell lysis via visual inspection and optical microscopy (VersaMax, Molecular Devices, Sunnyvale, CA, USA) observation and checked by measuring the absorbance at OD680. 340‚ 850 nm. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Whether its cell line development, 3D biology, drug screening—or a combination of the three—our technology maximizes throughput and reproducibility to give. The concentrations of anti-RSV G protein IgG antibodies in the samples were. New and used Molecular Devices VersaMax's for sale on LabX. StakMax Microplate Handling Stacker. To provide access to disconnect power from the instrument, maintain a 20 cm to 30 cm (7. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 0 out of 5 $ 23,995 $ 22,495. The SpectraMax Plus 384 Microplate Spectrophotometer is a versatile and reliable instrument that can measure absorbance in the UV and visible range. The colour change of the MTS solution was measured at 490 nm by using a tunable microplate reader (VersaMax Molecular Devices, USA). While the situation is vastly improving with numerous vaccine rollouts and over 6. Finally, the optical density of the wells was determined at an excitation wavelength of 450 nm and a reference wavelength of 540 nm on a VersaMax (Molecular Devices) microplate reader. Find Fanuc, Molecular Devices, and GE for sale on Machinio. The absorbance was read with a multiwall spectrophotometer (VERSAmax, Molecular Devices, Sunnyvale, CA, USA) at 450 nm. Technical Specifications. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. pneumoniae ATCC49619 was used as reference control strain as recommended (Clinical and Laboratory Standards Institute. The viability of the treated group was expressed as a percentage of non-treated control group, which was assumed to be 100%. StakMax. Plates values were acquired using a fluorescence microplate reader (570 nm; Versamax, Molecular Devices, San Jose, CA, United States). Although this manual has been prepared with every precaution to ensure accuracy, Molecular Devices SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. These values were transformed to logarithmic scale to create a log-log curve. 3 Methods 1. Wavelength Selection. Preparation of cytoplasmic and nuclear protein. Animals and preparation of tumor model. Concluding Remarks. Table of Contents. The RT-qPCR analysis was performed on a 7500 Fast Real-Time PCR system (Applied Biosystems, USA). Approximately. Cell viability was represented by percentage values compared to a. VERSAmaxTM Tunable Microplate Reader Operator s Manual for Instruments with Serial Numbers Beginning. At All-usermanuals. FAST AND ACCURATE METERTECH Microplate Reader $3,650. The culture supernatant of cells (25 μl) was mixed with 225 μl of DMMB dye solution and the absorbance of solution mixture was measured at 525 nm with a spectorophotometer (VERSAmax; Molecular Devices Japan KK, Tokyo, Japan). To measure the luciferase activity biochemically, cells wereThe activity was measured colorimetrically at 450 nm using a microplate reader (Versamax, Molecular Devices, Sunnyvale, CA, USA). Molecular Devices公司的VersaMax读 板机为光栅型单色器读板机，拥有灵活检 测优势，无需加配额外的滤光片。 可调波长，无需滤光片，灵活 VersaMax读板机使用光栅单色器来选择 波长，其结果是选择确切的检测波长应用 于每个实验。波长范围从340nm到With the VersaMax microplate reader, you will never have to buy another filter. Spectranet customer portalThe absorbance was recorded at 532 nm using a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, USA). Absorbance was read at 412 nm every minute for 10 min using a VersaMax (Molecular Devices, San Jose, CA) spectrophotometer. Analytical Technologies Group specializes in the maintenance and repair of Molecular Devices microplate readers, including the SpectraMax® and VersaMax™ absorbance readers. Values are presented as the mean ?standard deviation (SD). The absorbance of each well was measured at λ = 490 nm with a VersaMax (Molecular Devices, USA) microplate reader. With its advanced features and user-friendly interface, the VersaMax is the perfect choice for a wide range of research. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The value for non-specific absorption at 600 nm was subtracted. 8), 40 μL of tyrosinase, and 40 μL of L-DOPA was put in each well. ,, [28]. Furnace Manuals. Concentration was determined by BCA Protein Assay Kit (ref. Quantitative real-time polymerase chain reaction For quantitative real-time polymerase chain reaction (qRT-PCR) analysis, total cellular RNA was extracted from ten zebrafish larvae. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. Study conductThe amounts of chlorophyll a and chlorophyll b were measured at 664. Microplate Readers Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Ensure data accuracy of your reader. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time.